Search results for "microsatellite markers"
showing 10 items of 20 documents
Heterozygosity-fitness correlations in adult and juvenile Zenaida Dove, Zenaida aurita.
2013
10 pages; International audience; Understanding how fitness is related to genetic variation is of crucial importance in both evolutionary ecology and conservation biology. We report a study of heterozygosity-fitness correlations in a wild, noninbred population of Zenaida Doves, Zenaida aurita, based on a sample comprising 489 individuals (382 adults and 107 juveniles) typed at 13 microsatellite loci, resulting in a data set comprising 5793 genotypes. In both adults and juveniles, and irrespective of sex, no evidence was found for an effect of either multilocus or single-locus heterozygosity on traits potentially related to fitness such as foraging tactic, competitive ability, and fluctuatin…
Population genetic structure and milk production traits in Girgentana goat breed
2017
The aim of this work was to evaluate the genetic status of the Girgentana goat, an endangered breed from Sicily (Italy), using microsatellite markers. Furthermore, as the main purpose of the Girgentana breed is milk production, quantitative milk traits were investigated, including fatty acid profile. Molecular data from CSN1S1, CSN2, CSN1S2, and CSN3 casein genes were also used to infer haplotypes. A total of 264 individuals were collected. Samples of Maltese (n = 41) and Derivata di Siria (n = 33) goat breeds were also used to understand the genetic relationship among breeds. Test-day records for milk production were collected to determine daily milk yield, fat, protein, casein, lactose, a…
Les ambroisies annuelles (Ambrosia artemisiifolia et Ambrosia trifida) : réponse adaptative au désherbage chimique et connectivité des populations da…
2018
The primary aim of this work was to study the risk of resistance evolution to acetolactate synthase (ALS) inhibiting herbicides in mugwort (Ambrosia artemisiifolia L.) through four points: (i) selection pressure (study of the efficacy of a range of ALS-inhibiting herbicides), (ii) the adaptive response capacity of the weed (determination of the variation in sensitivity to ALS inhibitors between plants and implementation of a recurrent selection programme), (iii) a field study (search for resistance to ALS inhibitors in the field in France), (iv) the study of resistance mechanisms (target-linked - TLR - and non-target-linked - NLR - by a transcriptomics approach). The second objective was to…
Genetic and aromatic diversity of the Burgundy truffle
2013
Truffles are ectomycorrhizal Ascomycota fungi belonging to the Tuberaceae family and more specifically to the Tuber genus. More than thirty Tuber species are currently described in Europe. In this thesis, we specifically focused on the Tuber aestivum-uncinatum model. This truffle is commonly called "Burgundy Truffle" and has a gastronomic and cultural interest.The first part of this thesis focused on the taxonomic status of the Burgundy truffle (Tuber uncinatum). For this, we used a multi-marker approach combining several genetic markers commonly used at the interspecific scale. Our analyses showed that the two taxa, Tuber aestivum (summer truffle) and Tuber uncinatum are conspecific.In the…
Agroecosystems shape population genetic structure of the greenhouse whitefly in Northern and Southern Europe
2014
International audience; Background: To predict further invasions of pests it is important to understand what factors contribute to the genetic structure of their populations. Cosmopolitan pest species are ideal for studying how different agroecosystems affect population genetic structure within a species at different climatic extremes. We undertook the first population genetic study of the greenhouse whitefly (Trialeurodes vaporariorum), a cosmopolitan invasive herbivore, and examined the genetic structure of this species in Northern and Southern Europe. In Finland, cold temperatures limit whiteflies to greenhouses and prevent them from overwintering in nature, and in Greece, milder tempera…
Centennial olive trees as a reservoir of genetic diversity
2011
†Background and Aims Genetic characterization and phylogenetic analysis of the oldest trees could be a powerful tool both for germplasm collection and for understanding the earliest origins of clonally propagated fruit crops. The olive tree (Olea europaea L.) is a suitable model to study the origin of cultivars due to its long lifespan, resulting in the existence of both centennial and millennial trees across the Mediterranean Basin. †Methods The genetic identity and diversity as well as the phylogenetic relationships among the oldest wild and cultivated olives of southern Spain were evaluated by analysing simple sequence repeat markers. Samples from both the canopy and the roots of each tr…
The Sicilian peach (Prunus persica L. Batsch) germplasm: Evaluation of genetic diversity using SSRs
2006
The decrease in worldwide consumption of peaches due to the poor flavour quality of the fruit has increased the demand for new tasty cultivars. The availability of plant genetic resources is an important prerequisite for improving fruit quality by breeding. In Sicily there is a large collection of local cultivated peaches, whose production fills a niche in the local markets, but which is at risk of disappearing. Their characteristics include highly aromatic fruit and late ripening. Because of their important agronomic characteristics, the Department of Colture Arboree of Palermo University has collected the most interesting accessions to safeguard them and to use the indigenous resources in…
Recovery and genotyping ancient Sicilian monumental olive trees
2023
The long-lived and evergreen olive tree dominates the Mediterranean landscape, representing an agroecological and cultural symbol and a genetic heritage of inestimable value. Sicily, for historical, geographical, and cultural reasons, has a very rich and distinctive olive germplasm. In this work, a large survey was conducted to discover, collect, and characterize the genetic diversity of centennial monumental olive trees from historical sites, such as the Greek Temple Valley (Agrigento), ancient gardens, or farmland present in the western part of the island. Trees were chosen based on their height, trunk, stump size, and presumed age; particularly, only olive trees with an age estimated at …
Genetic variability of Girgentana goat breed using molecular markers
2014
Assessing genetic diversity of local breeds through the information provided by neutral molecular markers, allows determination of their extinction risk and to design strategies for their management and conservation. The aim of this work was to quantify the levels of genetic variability in Girgentana goat breed using microsatellite markers and genetic polymorphisms at the casein genes. A total of 264 individuals were genotyped for 20 microsatellites, selected as suggested by ISAG and FAO. Moreover, the same individuals were characterized for the casein loci (CSN1S1, CSN2, CSN1S2, and CSN3) using PCR and sequencing protocols. Several genetic diversity indexes were estimated. A total of 130 a…
Genetic characterization of the Mascaruna goat, a Sicilian autochthonous population, using molecular markers
2013
The aim of this work was to characterize a Sicilian autochthonous goat population using microsatellite markers and genetic polymorphisms at the casein genes. In order to investigate the genetic structure of the Mascaruna goat, a total of 60 (20 Girgentana, 20 mixed populations, and 20 Mascaruna) individuals were analyzed, using a panel of 18 microsatellite markers. Moreover, the Mascaruna goats were genotyped at casein loci using several molecular techniques. Based on the genetic structure at casein genes, the Mascaruna goat was similar to most goat breeds from the Mediterranean area, which are characterized by the predominance of strong alleles. The low value of genetic differentiation amo…